Journal: Frontiers in Genetics
Article Title: Analysis of the type II-A CRISPR-Cas system of Streptococcus agalactiae reveals distinctive features according to genetic lineages
doi: 10.3389/fgene.2015.00214
Figure Lengend Snippet: Genetic organization of the CRISPR1- cas locus in Streptococcus agalactiae . cas genes and core genes are shown as black arrows and blue arrows, respectively. The leader sequence is located between the cas gene cluster and the CRISPR array (white box; L) while the trailer sequence is located downstream of the array (white box; T). The direct repeats (DR) are shown as black diamonds and the terminal repeat, which differs from the consensus DR, is shown as a white diamond. Spacers are shown as colored rectangles and unique spacers are represented by unique colors. Below the CRISPR array, the sequence of the first two repeat-spacer units is shown with the DRs in black characters and the spacers in color characters.
Article Snippet: In a second step, CRISPR1 array structure was determined anew using a macro-enabled Excel tool named CRISPR database II (P. Horvath, DuPont) that comprises different programs for the identification and extraction of CRISPR features in nucleotide sequences, and for subsequently establishing a graphic representation of spacer diversity.
Techniques: Sequencing, CRISPR